Bowtie 1 was used to align the original reads for each tissue in the raw data files to the assembled Trinity transcripts. This generated bowtie.coordSorted.bam files that are used for visualizing using Integrated Genomic Viewer. For each tissue, you can choose to visualize the alignment in either the RF (reverse/forward) direction or the FR (forward/reverse) direction.
For more information about Bowtie 1, visit: http://bowtie-bio.sourceforge.net/index.shtml
For more information about IGV, visit: https://www.broadinstitute.org/igv/home